期刊名称：Asian Journal of Biological and Life Sciences
印刷版ISSN：2278-747X
电子版ISSN：2278-5957
出版年度：2013
卷号：2
页码：50-53
出版社：Pbspublishing
摘要：Three snake venoms: Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis were studied for cytotoxic activity and apoptosis on five cancer cell lines; BT474, SW620, KATO-III, Hep-G2 and ChaGo. Cytotoxic effect was determined by MTT assay and flow cytometry has been used for apoptosis. Various concentrations of three venoms showed cytotoxic against cancer cells as time-dependent. The potent of cytotoxic from C. albolabris venom against cancer cells were: BT474 (2.96 ± 0.44 µg/ml), SW620 (3.32 ± 0.14 µg/ml), KATO-III (3.72 ± 0.11 µg/ml) and Hep- G2 (3.74 ± 0.43 µg/ml), respectively. C. rhodostoma venom showed cytotoxic to BT474 (3.16 ± 0.69 µg/ml), SW620 (3.5 ± 0.01 µg/ml) and KATO- III (3.74 ± 0.37 µg/ml) while the venom of D. siamensis was highly toxic to only ChaGo (0.48 ± 0.04 µg/ml). Apoptotic cell death using PI staining was dose and time dependent.
