摘要:Preliminary phytochemical screening of Thespesia populnea demonstrated the presence of flavonoids, glycosides, saponins, alkaloids and triterpenes. Antibacterial activity of methanolic extract of Thespesia populnea was Checked at a lowest concentration by cup and plate method. Zones of inhibition produced by methanolic extract in a dose of 5, 10, 25, 50 and 100 μg/ml against some selected strains was measured and compared with those of standard antibiotic levofloxacin (10 μg/ml). Keywords: Thespesia populnea; antibacterial activity; cup and plate.