摘要：Purpose: We hypothesize that molecular changes leading to Osteoarthritis (OA) occur soon after a meniscal tear and long before the manifestation of OA. We sought to characterize the gene expression profile in synovial fluid at different time points following a meniscal tear with a focused examination on inflammatory and arthritis related genetic markers. Methods: Synovial fluid was collected from 8 male patients (24~48yrs old) with evidence of meniscal injury on Magnetic Resonance Imaging (MRI). Illumina humanht-12 microarray and RNA-Seq were used to characterize gene expression profile in cell pellets and supernatant, respectively. Unsupervised clustering analysis was performed to identify patterns of gene expression profile among samples. Metacore was utilized to perform pathway analysis. T-tests with False Discovery Rate (FDR) corrections were used to compare subset of genes among individuals. Results: Individuals with short injury duration (
