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  • 标题:A COMPARISON BETWEEN THE EFFECTS OF ALBENDAZOLE AND MEBEN DAZOLE ON THE ENZYMATIC ACTIVITY OF EXCRETORY / SECRETORY PROD-UCTS OF ECHINOCOCCUS GRANULOSUS PROTOSCOLECES IN VITRO
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  • 作者:ADNANI SADATI SEYED JAFAR ; FARAHNAK ALI ; MOLAEI RAD MOHAMMAD BAGHER
  • 期刊名称:Iranian Journal of Public Health
  • 印刷版ISSN:2251-6085
  • 电子版ISSN:2251-6093
  • 出版年度:2016
  • 卷号:45
  • 期号:2
  • 页码:223-229
  • 语种:English
  • 出版社:Tehran University of Medical Sciences
  • 摘要:Background: Hydatid cysts are formed in human body can be treated clinically by surgery or drugs such as albenda-zole (ABZ) and mebendazole (MBZ). The purpose of this study was comparing the effects of ABZ and MBZ on glu-tathione-S-transferase, alkaline phosphatase and protease enzymes activities in protoscoleces of hydatid cyst.
    Methods: The culture supernatants containing the parasite Excretory / Secretory (E/S) products were collected every 12 h for 72 h. The E/S products of treated samples with 1 m g/ml ABZ and MBZ and the control one were collected and after centrifugation then protein concentrations were measured according to Bradford method. GST, ALP and protease activities of E/S products were assessed photometrically.
    Results: The mean of GST specific activity level in treated protoscoleces with ABZ and MBZ and in control group were obtained 69.44, 132.83 and 225.47U/mg/protein/ml respectively. The mean ALP activity level in treated protos-coleces with ABZ and MBZ and in control group were detected 19.22, 22.27 and 27.85 U/mg/protein/ml respective-ly. The protease activity level in treated protoscoleces with ABZ and MBZ were not detected. While the mean of pro-tease activity level in control group was 7.61U/mg/proteins. Statistical analysis showed the significant difference be-tween protein concentrations, the specific activities of GST, ALP and protease enzymes in treated protoscoleces in comparison with control group (P<0.05). Also, the significant difference were seen between specific activities of GST and ALP enzymes in treated protoscoleces with ABZ in comparison with treated group with MBZ (P<0.05).
    Conclusion: ABZ is more effective on the enzymes activities (GST and ALP) as compared with MBZ.
  • 关键词:HYDATID CYST PROTOSCOLECES; ALBENDAZOLE; MEBANDAZOLE; PROTEASE; GLUTATHIONE S-TRANSFERASE; ALKALINE PHOSPHATASE
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