The southwestern parts of Kadapa district are highlighted in Fluoride contamination. The Groundwater is the primary source of drinking water in this area and very few people are fed with water supply scheme. Geologically the limestone is the most predominant rocks of the study area these rocks have fluoride bearing minerals which are leached out to the groundwater and contribute high fluoride concentration in the groundwater. Total fifteen water samples are collected from different locations of Muddanur area and tested in the laboratory using Ion-Selective Electrode method. Fluoride levels in 93% of samples exceed the maximum permissible limits (1.5 mg/L) set by the ISO and WHO. The observed Fluoride levels in this area range from 1.43–2.54 mg/L with an average of 2.07 mg/L. The high fluoride levels may lead to morbidity of dental fluorosis .It is finally concluded that the Muddanur need a sound Fluoride management plan and the removal of fluoride from drinking water is advisable.