标题：Isolation And Analysis Of Biodegradation Potential Of The Bacterial Endophytes Acinetobacter Beijrenckii, B5 And Enterobacter Aerogens, B8, Against Chlorpyrifos In Vitro And In Plantae
摘要：Chlorpyrifos is one of the most commonly used organophosphate insecticides that areimplicated in serious environmental and human health problems. Herein, we aim to isolate potentialbacterial strains that could metabolize chlorpyrifos. For this, we screened the endophytes from theroots of the strawberry plants that could metabolize and degrade chlorpyrifos (10 mg/L). Two strainsnamed B5 and B8 identified that grow at the expense of chlorpyrifos as the sole carbon source. B5 andB8 are able to degrade 100% and 90% chlorpyrifos (10 mg/L) in vitro within 72 h and 96 h respectivelyas tested spectrophotometrically and via Gas Chromatographic (GC) analysis. Furthermore, using peaseedlingas model, our in plantae experiments show that chlorpyrifos taken up by the seeds can beefficiently degraded by strains B5 and B8 as reckoned by Gas Chromatographic (GC) analysis. Boththe strains, B5 and B8 are able to degrade 97% and 88% of chlorpyrifos (10 mg/L) within 24 h of thetreatment. 16S rRNA gene sequencing revealed that strain B5 showed 99% identity with Acinetobacterbeijrenckii strain MP17_2B and strain B8 also shared 99% identity with the Enterobacter aerogensstrain BPRIST043. Additionally the strains also possess biofertilzer activities like phytate production,ammonia production and nitrogen fixation. The results strongly suggest that these strains possessuseful bioremediation activities which can be used to improve the quality of food products.
