This contribution summarises the most important results of the environmental methods applied in a study of a significant hillfort at Vrcovice (South Bohemia) from the beginning of the Middle Bronze Age. Attention was already paid to the environmental attributes of the archaeological sources ( e.g. phosphate analysis) during systematic excavations over the years 1963–1966. Important findings emerged out of archaeo-environmental research conducted here in 2013, during which a number of methods were applied to study the economic system (archaeozoology, food detection by antibodies, plant macro-remains), the natural environment (anthracology, palynology) and the reconstruction of the original form of fortification (magnetic susceptibility, remanent magnetization). Samples were also obtained for two radiocarbon dates. The hillfort at Vrcovice ranks among the most important resources for the study of fortified settlements from the Bronze Age in Bohemia due to the holistic approach taken.