摘要：Two Ganoderma species causing butt rot were collected and isolated from Ficus bengalensis tree at Mansoura and Citrus limon trees at Damietta, Egypt. The two species were identified, based on fruit bodies, culture characterization and taxonomic traditional methods. The DNA sequences analysis of the ribosomal 5.8S rRNA gene including the flanking internal transcribed spacers (ITS) was also performed to confirm their taxonomic position among the other Ganoderma species. The species status was confirmed as G. resinaceum EGM and Ganoderma sp EGDA. For conserving the two Ganoderma species, they were cultivated in sawdust bags of citrus, beech and spruce trees for the first time in Egypt. Primordial initiation and basidiocarp maturation were observed as the sequence of fruit body development in both species. The antler–like formation was only observed in Ganoderma sp EGDA.
关键词：Ganoderma – culturing – sawdust – phylogenetic tree – ITS DNA sequencing
