摘要:In this study, 71 strains of yeast were isolated from Piper betle and P. nigrum . Isolates were identified using sequence analysis of the D1/D2 region of large 26S ribosomal subunit rDNA. They belong to 25 species in 11 genera. Strains Cryptococcus luteolus InaCC Y-265, Candida orthopsilosis InaCC Y-302, and Candida oleophila InaCC Y-306 could accumulate more than 40% of lipid per g of cell biomass on a dry weight basis. The fatty acids observed were primarily palmitic acid (C18:1), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1) and linolenic acid (C18:2). The fatty acid profiles suggest that these yeasts may be good candidates for biodiesel production, as they are similar to the fatty acids of plant oils currently used for biodiesel.