摘要：Phytoadditives have gained increasing interest as feed additives for fish. The aim of the present study was to determine whether selected dietary phytoadditive can influence the bioavailability of several trace elements (Cu, Mn and Zn), which play an important role in the physiological processes. The experiments were carried out at a commercial trout farm. A total of 420 juvenile rainbow trout (mean weight ± SD = 10.79±0.57), Oncorhynchus mykiss, were randomly allocated into four different treatments with three replicates each. Fish were kept in raceways (3X0.8X0.4 m) at 10±1°C with a natural photoperiod. Proper amount of carvacrol was sprayed on 1 kg of commercial trout diet to prepare four diets with 0 (Control, C0), 1 (C1), 3 (C3) and 5 (C5) carvacrol g/kg diet. Fish were fed to apparent satiation three times per day. The feeding trial lasted four weeks. Then, in different type of fish tissues (muscle, liver and pyloric caeca) from fish fed with diets enriched in carvacrol, beneficial elements (Cu, Mn and Zn) were analysed by atomic adsorption spectrophotometer. Results showed that the levels of Cu, Zn and Mn were especially significantly increase by C1 diet in all tissues (muscle, liver and pyloric caeca) except muscle and pyloric caeca Zn. The results of this experiment indicate that the carvacrol had the ability to potentiate the trace element retention. Although bioaccumulations of Cu, Zn and Mn in the muscle, liver and pyloric caeca are well demonstrated, the exact mechanisms of phytoadditives are still only partially understood. More investigations are required to detail the mechanisms involved in phytoadditives this enhancement.
