摘要：The neutrophilic iron-oxidizing bacteria are different in morphology, physiology and taxonomic status but are united by their ability to oxidize Fe2+ at neutral pH and to form insoluble ferric oxides/(oxy)hydroxides. The hydroxides formed on their sheaths are of great interest for application in different nanotechnologies, biomedical and bioengineering applications. The bacteria are typical oligocarbophils which makes them a successful indicator for organic pollution of the water. The main problem is connected with the formation of sheaths. The goal of this study is connected with the optimization of the cultivation conditions to obtain sheaths with ferroxides of neutrophilic iron bacteria. The optimization included investigations of the types of cultivation, used of the different nutrient media and the duration of the cultivation.The analysis of the results shows that the isolates grow poorly at 10°С and 37°С. Optimal growth of the cultures was observed at 20оС under dynamic conditions. The most appropriate for the formation of sheaths was SIGP medium. The formation of the sheaths started after 7 days cultivation period.The analysis of the ferroxides formed shows that the composition of the nutrient medium strongly influences the type of the formed ferroxides.
关键词：neutrophilic iron bacteria; sheathed bacteria; Leptothrix; nanotechnologies
