摘要：The aim of the study presented was to evaluate the influence of a newly synthesized strontium-modified dicalcium phosphate dihydrate (DCPD) (CaHPO4.2H2O, brushite) on viability and proliferation of murine fibroblasts (BALB/c 3T3) and cultures from bone explants. The investigations were performed by thiazolyl blue tetrazolium bromide (MTT) test, neutral red uptake cytotoxicity assay (NR) and crystal violet staining (CVS). The cells were cultured in DMEM medium incubated for 4 h in the presence of the examined calcium phosphate placed on glass slide (100 mg compound/5 cm2 glass slide, 10 ml medium). The results obtained revealed a high survival rate of the cells after 72 h treatment period. A positive correlation between the data coming from cytotoxicity assays with different cellular targets and mechanisms of action was observed in BALB/c 3T3 cells - NR (100.4% ± 5.5), CVS (113.4% ± 5.2) and MTT (120.5% 11.1). In addition, very close results were obtained by MTT test for BALB/c 3T3 fibroblasts (120.5% ± 11.1) and cultures from bone explants (116.7% ± 5.6). The luck of toxicity for the cells used as model systems in our study indicates that the tested material is a promising candidate for further in vivo investigations.
