摘要：The aim of the study presented was to evaluate the effect of ammonium vanadate (NH4VO3) on viability and proliferation of cultured tumor and non-tumor cells. Permanent cell lines obtained from some of the most common human cancers were used as model systems: MCF-7 (breast cancer), HeLa (carcinoma of the uterine cervix), HepG2 (hepatoma). The non-tumor cell lines established from human embryos (MRC-5 and Lep-3) and bovine kidney (MDBK) were also included in the experiments. The investigations were performed by thiazolyl blue tetrazolium bromide (MTT) test, neutral red uptake cytotoxicity assay (NR) and crystal violet staining (CVS). The results obtained revealed that applied at concentrations of 0.1, 0.5, 1, 5, 10 and 20 μg/ml for 24 h, 48 h and 72 h, NH4VO3 decreased significantly (in a time- and concentration-dependent manner) the viability and proliferation of human tumor (MCF-7, HeLa, HepG2) and non-tumor (MRC-5, Lep-3) cells whereas bovine kidney MDBK cells seem to be relatively more resistant. A positive correlations between the data coming from MTT, NR and CVS methods were observed.
关键词：Ammonium vanadate; tumor and non-tumor cell lines; cytotoxicity assays
