标题：Chemoprotective, antioxidant and immunomodulatory in vitro -effects of Aronia melanocarpa total extract on laboratory-cultivated normal and malignant cells
摘要：Chemoprotective influence of Aronia melanocarpa total extract on the side effects of the commercial chemotherapeutic drug Doxorubicin on laboratory-cultivated normal and malignant cells was studied. For this goal, morphological changes in both used cell types separately and in mixed cultures of them in the presence and absence of Doxorubicin; of Aronia-extract, were investigated. One of the main mechanisms of Aronia polyphenols and anthocyanins action has been supposed to be by increase of the reduced Glutathione (GSH) intracellular levels. According our results, strong cytotoxic effect of Doxorubicin on in vitro-cultivated normal and malignant cells was observed, but in the presence of Aronia-extract, regeneration in the vitality and even in the proliferation capacity of both cell types was indicated. Furthermore, a strong myeloid cell differentiation of both normal and malignant cells was indicated by the Aronia-extract, which was strongest in normal cells, co-cultivatied with myeloma cells, independently of Doxorubicin presence or absence. The observed contradictory results in GSH levels regeneration in the presence of Aronia-extract could be explained with a partial depletion of intracellular GSH in the concrete time of experiment, probably on the basis of feed-back principal of regulation, despite the eventual previous increase in its levels on the influence of Aronia-extract, in agreement with the respective literature data. Thus, further studies, directed to investigation on the influence of the total Aronia-extract, but also of its separate components (polyphenols and anthocyanins) on the levels of intracellular GSH, should be necessary, as well as, on the other hand, on the influence of intermediate cell components as GSH, on the processes of cell growth and proliferation by cascade regulatory pathways, are necessary
关键词：Aronia-extract; Doxorubicin; normal cells; malignant cells; GSH; cascade regulatory pathways
