标题：Investigation of organs allocation and shift of ascorbate radicals levels in different organs by Cynara scolymus Linn. extracts - real time electron paramagnetic resonance study
摘要：A great number of spicy, medicinal and other plants contain chemical compounds exhibiting antioxidant properties. Cynara scolymus Linn. plant, cultivated in the Mediterranean regions and central Europe also known as globe artichoke is a perennial, frost sensitive, thistle-like plant belongs to the family Compositae, demonstrated exellent in vitro/ ex vivo antioxidant properties and strong radical scavenging capacity. By the present study for the first time using EPR spectroscopy we report our investigations on the ‘’real time’’ level of ascorbate radicals and compound- allocation in the livers and in the kidneys of mice after administration of extract from seeds of Bulgarian Cynara scolymus Linn. White non-inbred laboratory mice were inoculated i.p.: first group with Cynara scolymus Linn. (40 mg/kg) and the second control group was inoculated with the solvent only. The mice were dissected at the different time intervals (10 min – 24 hrs) post injection and fresh tissues from liver and kidneys were homogenated in cold PBS solution and tested for organ allocation. Tissue homogenates in DMSO was prepared for determination of the ascorbate radicals (Asc.) level. Maximum concentrations in livers and kidneys were established at 60th min after administration of Cynara scolymus Linn. In tested group with Cynara scolymus Linn., the level of ascorbate radicals in the livers and in the kidneys were lower than that measured in the control group. In conclusion, these first Electron Paramagnetic Resonance investigations characterize Bulgarian Cynara scolymus Linn. as a promising natural antioxidant
