期刊名称：Studia Universitatis Moldaviae: Stiinte Exacte si Economice
印刷版ISSN：1857-2073
电子版ISSN：2345-1033
出版年度：2007
期号：7(07)
页码：62-67
出版社：Moldova State University
摘要：Monitoring of the seed quality at different stages of plant breeding at sunflower represents a very important elementin obtaining high yield hybrids based on CMS-Rf genetic system. Sunflower represents a protandric plant with a singleterminal inflorescence which contains 700-3000 individual hermaphrodite flowers, exposed on the calathidium, makingmanual emasculation almost impossible. That’s why the utilization of the male sterility was indispensable and necessaryfor the obtaining of the high level of hybridization at all the stages of sunflower breeding. Wide utilization of maternallines, with cytoplasmic male sterility, and of paternal lines, with fertility restoring genes Rf, lead to the necessity toobserve the stability and homogeneity of parental genotypes. Thus, for the paternal genotypes it is important to verify thepresence and the stare of Rf genes, but maintaining male sterility is verified by the level of sterility control. Utilizationof PCR-based methods with specific primers allows controlling genotype quality
