摘要：Oxidative stress appears as the key feature associated with dysfunction in adipose tissue and a major factor in the mechanisms of altered lipid metabolism in obesity. Cellular response of adipocytes in the conditions of oxidative stress results in maintaining systemic pro-inflammatory state, insulin resistance and increased accumulation ofvery long-chain saturated fatty acids (VLCSFAs) to the liver, which are lipotoxic and lead to further injury. Therefore, the therapeutic purposes of lowering the production of ROS, may have beneficial effects on obesity and its associated complications. The aim of the study was to determine the influence of allopurinol (xanthine oxidase inhibitors) on oxidative stress in adipose tissue and liver saturated fatty acids content in a model of fructose-induced obesity. We used a model of high-fructose diet (HFD) in male rats Wistar (16 weeks, 35% glucose-fructose corn syrup), divided into three groups: control; HFD; HFD and allopurinol administration (150 mg/kg in drinking water for 16 week). Analysis of fatty acids was performed by Gas Chromatograph with MS detector. Serum levels of glucose and uric acid (UA); weight, markers of oxidative stress- MDA (malondialdehyde), glutathione (GSH) and glutathione peroxidase (Gpx) in the retroperitoneal tissue were investigated. The results showed significantly elevated of VLCSFAs, retroperitoneal tissue/ body weight ratio, MDA, Gpx, glucose and UA levels in serum and decreased levels of glutathione in HFD rats compared to the control group. In the group treated with allopurinol the retroperitoneal tissue/ body weight ratio, the levels of MDA, Gpx, VLCSFAs, UA and glucose levels in serum were significantly reduced while glutathione levels were elevated in comparison with HFD rats. The inhibition of xanthine oxidase and UA by allopurinol prevents the development of oxidative changes in adipose tissue. This effect probably suppresses inflammation in adipose tissue, improves insulin sensitivity, reduce VLCSFAs levels and thereby prevent the further lipotoxicliver damage
