摘要：The influence of different nitrogen sources (ammonium nitrate, urea and ammonium nitrate + urea) and different concentration of the medium, containing both nitrogen sources on the growth and the content of pigment, protein, carbohydrate and lipid of a newly isolated green alga Scenedesmus sp. BGP was studied. Even though the two nitrogen sources were present in the medium routinely used in the lab, the experiments showed that the use of medium with each one of them separately gave a better yield of algal biomass for the whole cultivation period. The best growth was observed in urea-containing medium, where the dry weight reached 9.0 g/l. Dilution of the standard medium (2-, 4- and 8- fold) had positive impact on the growth, in contrast to 2-fold concentrated. The total amount of pigments (chlorophyll a+b and carotenoids) correlated with the change of growth in the different media. As a whole, carbohydrates, followed by proteins and lipids, dominated the biochemical composition of Scenedesmus sp. BGP. The biomass of the alga, grown in urea- or ammonium nitrate-containing medium was characterized by stable qualitative content over the entire period of cultivation (about 29% proteins, 41% carbohydrates and 24% lipids). These results showed that the medium with urea was most suitable for cultivation of Scenedesmus sp. BGP, because as a nitrogen source, urea is more efficient in terms of productivity and is less expensive
