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  • 标题:Unusual behavior of natural polyphosphates during IMAC
  • 本地全文:下载
  • 作者:Kmínková M. ; Prošková A. ; Kučera J.
  • 期刊名称:Czech Journal of Food Sciences
  • 印刷版ISSN:1212-1800
  • 电子版ISSN:1805-9317
  • 出版年度:2001
  • 卷号:19
  • 期号:5
  • 页码:161-165
  • DOI:10.17221/6601-CJFS
  • 出版社:Czech Academy of Agricultural Sciences
  • 摘要:The atypical course of IDA-ferric (Imino-Di-Acetyl-agarose saturated with ferric ions) of two polyphosphorylated compounds – phytic acid and Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster obtained by the way of pancreatic hydrolysis of bovine casein was detected. The spectral data (for phytic acid) and iron content (for polyphosphate cluster) indicate the strong bond of ferric ions to these polyphosphorylated compounds. The data shows these compounds to bind ferric ion stronger even in comparison with IDA (Imino-Di-Acetate) itself. Therefore attention is to be paid to all IMAC (Immobilized-Metal-Ion-Affinity-Chromatography) of natural compounds containing strong chelating groups or several chelating groups in vicinity.
  • 关键词:phytic acid; Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster; affinity chromatography; immobilized metal ion; IMAC; IDA-ferric; atypical adsorption
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