期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2016
卷号：113
期号：12
页码：3159-3163
DOI：10.1073/pnas.1524864113
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We use 1H NMR to probe the energy landscape in the protein folding and unfolding process. Using the scheme ⇄ reversible unfolded (intermediate) → irreversible unfolded (denatured) state, we study the thermal denaturation of hydrated lysozyme that occurs when the temperature is increased. Using thermal cycles in the range 295
关键词：protein folding ; proton NMR ; energy landscape ; hydration water
