In present research exploration, sugar cane bagasse an indigenous waste/by product carbon source was tested as substrate for optimum biosynthesis of xylanase by Aspergillus niger using the submerged fermentation technique. The for xylanase production, thee concentration levels (2.5, 3.0 and 3.5%) of sugar cane bagasse, four different fermentation temperatures (i.e. 25.0, 27.5, 30.0 and 32.5oC) and four initial pH levels (5.0, 5.5, 6.0 and 6.5) were used for the culture medium for a period of 24, 48, 72, 96 h. The results revealed that the Aspergillus niger exhibited maximum enzyme activity (44.001.45) at 3.0 % sugarcane bagasse concentration followed by 42.000.29 and 39.070.55 at 3.5 and 2.5 % concentration respectively at 30oC, 5.5 pH after a period of 72 h of incubation. The comparison of the effect of various initial pH levels of culture medium exhibited that pH 5.5 had most potent role in xylanase synthesis as compared to different other pH levels. Time scale analysis revealed that fermentation period of 72 h was the most suitable for obtaining maximum enzyme activity. Moreover a temperature of 30oC was found to be optimum for higher yields of xylanase. Conclusively, for optimum production of xylanase production 3.0% sugarcane bagass was used in the culture medium having pH 5.5 for the period of 72 h at 30oC.