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  • 标题:Immunological response of Lampito mauritii (Kinberg) coelomic fluid determined by hemolytic activity and sperm cytolysis
  • 本地全文:下载
  • 作者:J. Marlin Cynthia ; K. Uma ; R. Gouthami
  • 期刊名称:International Journal of Innovative Research in Science, Engineering and Technology
  • 印刷版ISSN:2347-6710
  • 电子版ISSN:2319-8753
  • 出版年度:2014
  • 卷号:3
  • 期号:1
  • 页码:8238
  • 出版社:S&S Publications
  • 摘要:Earthworms have proved to be excellent inexpensive animals for studying regeneration, aspects ofneurophysiology, endocrinology and excretion. The body cavity of annelids contains coelomic fluid (CF), in whichcoelomocytes, the worm’s leukocytes, are suspended. The component of CF of Earthworm defenses them in identifyingforeign substance and develops potency to fight against them. The present study is the focused on the lytic effect of CFof Lampito mauritii on the vertebrate blood and sperm would pave the way to trace the immune phylogeneticsignificance. The Coelomic Fluid (CF) was collected and Hemolytic activity was determined and cytolysis of spermswas observed. The results of the present study reveal that the coelomic fluid is 100% haemolytic to the RBCs of fish,frog, calotes, goat and human. The coelomic fluid serially diluted showed gradual reduction in the hemolytic activity. Itis evident that cytolysis and Haemolysis are the properties of coelomic fluid proteins which act as the humoral defenseproteins to fight against invading antigenic cells. The coelomic fluid is a fluid system which allows these proteins tooefficiently interact with the foreign cells, either bacteria or any vertebrate blood cells and even sperms.
  • 关键词:Earthworm; Lampito mauritii; Coelomic fluid; Hemolytic activity; Cytolysis
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