摘要：The aim of this study was to verify the relationship between somatic cell counts (SCC) and the DNA yield extracted directly from buffalo milk. Twenty-five Murrah buffalo milk samples were measured SCC and extracted DNA. Milk SCC was between 16,000 and 1,934,000 cells/mL. DNA yield was within the range from 3747.2 ng/μl to 7080.7 ng/μl. The ratio of A260/A280 and A260/A230 of genomic DNA was ranged from 1.66 to 2.17 and 1.71 to 2.13, respectively. The results indicated that DNA can be extracted directly from Murrah Buffalo milk, but may be presence of some contaminants. The Pearson’s correlation analysis showed a very strong positive correlation between the DNA yield extracted directly from buffalo milk and the milk SCC (r = 0.927, P < 0.001). Milk sample was preferred to blood as a source of DNA, due to milk collection was routinely performed, as well as less expensive and more easily accomplished than blood collection. Moreover, milk collection was also less stressful to animals, given that capture, handling, and venipuncture were not required for milk sampling
