摘要:In this work we focused on the synthesis of new Azo Schiff bases which were prepared by condensation of 3-formyl-4-hydroxy phenylazo benzene. With both 1,3-diamino xylylene, 2,6-diamino Pyridine, 1,5-diamino-2-methyl pentane respectively. The Schiff bases were checked by different spectral technique (LC-MS, GC-MS, 1H-NMR, IR). The new Azo Schiff Bases were studied for antibacterial activities against (Bacillus subtilis and Staphylococcus aureus) are Gram positive and (Salmonella typhi and Esche-richia coli) are Gram negative. The azo compound ligand were exhibited a variable activity of inhibition on the growth of the bacteria