摘要：Pesticides are extensively used in the modern agriculture as insecticide due to their high efficiency. Unfortunately these compounds have acute toxicity and can harm to human health and the environment. So in furtherance of environment protection purposes and to secure human health safety it is necessary to develop the sensitive and rapid technologies for detection of pesticides residue. Biosensor can be replaced by today analyses methods like liquid chromatography, gas chromatography and enzyme -linked immune absorption measure by simplifying or removing sample preparation in result of decrease in analysis time and cost. Among these sensors enzyme based electrochemical biosensors are absorbing especially due to some specifications like high sensitivity, rapid reaction and miniature size that presented them as a promising alternative for rapid detection of pesticides. In this research we have provided possibility to measure and detect concentration of paraoxon poison through chemical electrode by Modifying gold electrode level with copper oxide nanoparticles and acetylcholinesterase and also we have studied the electrochemical behavior of this protein structure. We studied copper oxide nanoparticles synthesized in the laboratory chemically by using the spectrums Uv-vis, TEM, XRD and SEM and was confirmed by using the spectrums. Our synthesized nanoparticles are copper oxide and spectrophotometer device showed absorption in the 270 nm area for nanoparticles. It was determined by using TEM and SEM that our nanoparticles are in the form of circle and their size is 20-40 nm. Direct electrochemistry of the acetylcholinesterase was obtained in the gold electrode and analyzable and clear peaks were presented. Enzyme inhibition by paraoxon shows linear performance of its concentration in two ranges of 0.1-1.80 and 3-10 mmol. This electrochemical process is performed in phosphate buffered saline and in PH=7. This biosensor can be used to determine paraoxon due to its suitable characteristics and easy preparation
