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  • 标题:DESIGN A HYDROGEN PEROXIDE BIOSENSOR BY USE OF CATALYSES AND MODIFIED GLASSY CARBON ELECTRODE WITH CADMIUM OXIDE NANO PARTICLES AND ENZYME CATALYSES
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  • 作者:Mohammad Fazilati ; Saeid Rezaei Zarehi ; Hossein Salavati
  • 期刊名称:Journal of Current Research in Science
  • 印刷版ISSN:2322-5009
  • 出版年度:2014
  • 卷号:2
  • 期号:5
  • 页码:649-650
  • 出版社:Islamic press
  • 摘要:In this research we have identified new biosensor to measure hydrogen peroxide using catalase enzyme and glassy carbon electrode. Electrode modified by scanning electron microscope and transmission electron microscope was surveyed. Direct electrochemistry of Catalase in glassy carbon electrode was obtained easily and a couple of similar returnable peaks was presented from iron II and III with formal potential (E) about 48mV/ Ph that shows linear relationship between formal potential of the mentioned electrode and Ph between 5-11. This electrochemical process was performed in Phosphate buffered saline in Ph=7 and scanning speed of 100Mv/s . Designed sensor has high sensitivity and short responding time and can be used to determine concentration of hydrogen peroxide in linear range of 50-350 mmol. Also this sensor has very good stability. We synthesized the cadmium Nanoparticles chemically in laboratory and in the next stage studies of these Nanoparticles using x-ray diffraction (XRD) approved the claim that synthesized nanoparticles are cadmium oxide. Uv-vis spectrum of cadmium oxide nanoparticles showed absorption in 380nm area.
  • 关键词:Catalase Enzyme; Cadmium Oxide Nanoparticles; Glassy Carbon Electrode; Hydrogen Peroxide.
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