摘要：Alcoholic toxicity and the ethanol in vivo content in increased levels result in severe metabolic and behavioral perturbations. Thus, design and development of a biosensor for more precise and prompt detection of which attributed to available methods is of great importance. In the present study, developement of ethanol biosensor by Horseridash peroxidase and modified electrode with MgO nanoparticles were investigated. Electrochemical studies were carried out on basis of cyclic voltammogram method. A triple electrode system was used in electrochemical cell in a way that bare or modified carbon paste electrode with MgO nanoparticles was applied as working electrode; Ag/AgCl electrode was used as reference electrode and the platinum one as the counter electrode. Designed biosensor was able to detect ethanol within the range of 5 to 60 M. As the modified carbon paste electrode with MgO nanoparticles and HRP located at 4˚C, it maintained its initial activity by 92% in a 21-day period.
