期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2016
卷号：62
期号：2
页码：130-133
DOI：10.3177/jnsv.62.130
出版社：Center for Academic Publications Japan
摘要：The biosynthetic pathway of the pyrimidine moiety of thiamin was studied in the archaean Halobacterium salinarum . Thiamin is biosynthesized from 4-amino-5-hydroxymethyl-2-methylpyrimidine (pyrimidine) and 5-(2-hydroxyethyl)-4-methylthiazole (thiazole). The pyrimidine and the thiazole are biosynthesized de novo in microorganisms. The biosynthetic routes of pyrimidine in microorganisms differ between eukaryote and eubacteria. In the eukaryote Saccharomyces cerevisiae , histidine and pyridoxine are the precursors of pyrimidine, while in the eubacterium Escherichia coli , pyrimidine is biosynthesized from 5-aminoimidazole ribonucleotide (AIR), an intermediate of purine biosynthesis. Tracer investigations revealed that [15N]-, [1-13C]- and [2-13C] glycine, precursors of AIR, were incorporated into the pyrimidine in H. salinarum . These results suggested that the biosynthetic route of the pyrimidine in H. salinarum is similar to that of E. coli .
