期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：3
页码：10084
出版社：S&S Publications
摘要：The present study was carried out to evaluate the antioxidant and free radical scavenging activity ofethanolic extract of ZingiberofficinaleRhizomes (Zingiberaceae) in various systems. DPPH radical, superoxide anionradical, nitric oxide radical and hydroxyl radicalscavenging assays were carried out to evaluate the antioxidant potentialof the extract. The antioxidant activity of ethanolic extract increased in a dose dependent manner. About 50,100, 250and 500 μg of ethanol extract of Zingiberofficinale(EZO) showed 61.44,66.25, 72.01 and 76.85% inhibitionrespectively on peroxidation of linoleic acid emulsion. Like antioxidant activity, the effect of EZO on reducing powerincreases in a dose dependentmanner. In DPPH radical scavenging assay the IC50 value of the extract was found to be168.09μg/ml. EZO was found to inhibit the nitric oxide radicals generated from sodiumnitroprusside. The IC50 valuewas found to be 83.365 μg/ml, whereas the IC50 value of curcumin was 20.34 μg/ml. Moreover, the EZO was found toscavenge the superoxide generated byPMS/NADH-NBT system. EZO was also found to inhibit the hydroxyl radicalgenerated byFenton's reaction, where the IC50 value of EZO was found to be more than 1000 μg/ml and for catechinthe IC50 value was found to be 5 μg/ml, which indicates the prooxidant activity of EZO. The amounts of total phenoliccompounds were also determined in this study. Theresults obtained in the present study indicate that the EZO can be apotential source of naturalantioxidant.
