期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：3
页码：10091
出版社：S&S Publications
摘要：Postprandial hyperglycemia is a prime characteristic of diabetes mellitus and has been a focus in the therapyfor diabetes. One of the therapeutic approaches which involve decreasing hyperglycemia aims at inhibiting theenzyme α-amylase. The leaves of T. Cordifolia were studied for the presence of amylase inhibitors. In this study area,herbal remedies are considered convenient for management of Type 2 diabetes with postprandial hyperglycemia due totheir traditional acceptability and availability, low costs, lesser side effects. Comparative evaluation of alpha amylaseinhibitory activities of selected plants extracts. The extracts obtained were subjected to in vitro alpha amylaseinhibitory assay using starch azure as a substrate and porcine pancreatic amylase as the enzyme The fractions obtainedby successive fractionation using solvents of varying polarity were studied for the presence of primary and secondarymetabolites. The total phenolic content of the different fractions was determined by HPLC and was correlated withtheir amylase inhibitory potential. Similarly, the protein content of the extracts was also estimated to understand thenature of the inhibitor present. This study shows that the leaves of T. Cordifolia were effective in inhibiting α-amylase,thereby proving to be potential anti-hyperglycemic agents.
