期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：4
页码：11338
出版社：S&S Publications
摘要：Superoxide dismutase (SOD) is one of the major classes of antioxidant enzymes, which protects the cellularand subcellular components against harmful reactive oxygen species. In maize, three types of SODs are present basedon their constituent metal ions, namely Cu /Zn-SOD, Mn-SOD and Fe-SOD. In this study we critically assess thephylogenetic relationship and structural models for maize Cu/Zn SOD, Mn SOD and Fe SOD. The phylogeneticanalysis showed that Mn-SOD and Fe-SODs in maize have a greater degree of similarity between them as compared toCu/Zn-SODs. The secondary structure of Mn-SOD and Fe-SODs demonstrated similar characteristics of helices,sheets, turns and coils revealed that Mn-SOD and Fe-SOD were closely related, whereas Cu/Zn-SOD evolvedindependently. We found Mn-SOD and Fe-SOD had structurally analogous beta sheets. Homology modeling-enabledthree-dimensional structure prediction helped to understand the molecular functions of SOD proteins in improvingtolerance to oxidative stress in maize.
关键词：Maize; superoxide dismutase; phylogenetic analysis; Protein modelling; secondary structure
