期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：5
页码：12678
出版社：S&S Publications
摘要：Regeneration through mature cotyledonary node has set rapid regeneration of plants directly from explantswhich is more time-saving and presented as an effective strategy so we have developed regeneration protocol throughsingle shoot using cotyledonary node a rapid and efficient protocol for three Indian soybean cultivars. Two explantswere collected from single cotyledonary node and cultured on medium containing N6-benzylaminopurine (BAP) forgermination, BAP and indole-3-butyric acid (IBA) for shoot induction, Gibberellic acid (GA3) for shoot elongation andIBA for rooting of explants. The best combination of hormones for all genotypes were obtained as germination of seedson half B5 medium supplemented with 1.0 mgl-1 of BAP, shoot induction on full B5 medium having BAP 1.0 mgl-1 andIBA 0.2 mgl-1 and shoot elongation on GA3 0.75 mgl-1 in full MS medium. Under these conditions, the plantlets couldbe raised within 40-45 days. It was observed that selection of proper medium for regeneration of soybean can overcomegenotype associated problems. This regeneration system can be used for soybean transformation.
