标题：Evaluation of Lipase Production from A. Japonicus Using Various Vegetable Oil Substrates and Physico-Chemical Factors Affecting Lipase Activity by Response Surface Methodology
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：6
页码：13610
出版社：S&S Publications
摘要：Lipases from plant, animal and microbial sources are of great importance in industrial applications owingto their varied properties and functions. In this study, Aspergillus japonicus MTCC 1975 was cultured in a productionmedium containing Malt extract (20g/L), Wheat mill bran (10g/L), Soy flour (5g/L), and Whey (1%) for production oflipase varying the design variables pH, temperature and oil substrate concentration. After 72 hours of incubation in theproduction medium, the pseudomycelium was separated and the culture supernatant was assayed for enzyme activity.Initial experiments were done with ten different vegetable oils, varying pH and temperature on one variable at a timemethod. High yields of the enzyme and the enzyme activity were obtained with olive and sesame oils as substrate inducersin the medium. Further experiments with olive oil substrate were carried out based on three level full factorial design ofresponse surface methodology and the optimal values of the variables were identified by solving the regression equationsusing Matlab. Experimental results demonstrated that pH of 6, temperature of 300C and olive oil substrate concentration of1% are significant for optimal production and activity.
关键词：Lipase; A. japonicus; Vegetable oils; Physico-chemical factors; Response surface methodology
