期刊名称:International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN:2347-6710
电子版ISSN:2319-8753
出版年度:2014
卷号:3
期号:6
页码:14126
出版社:S&S Publications
摘要:This study investigates the prevailing seasonal changes of physico-chemical and microbial communityfor mesophilic bacteria and fungi at different degradations stages of municipal solid wastes. The samples werecollected from Excel plant (Vidyaranyapuram, Mysore) in different depths of pile during summer, rainy and autumnseasons in the year of 2011 to 2012 at once in 10 days intervals up to 60 days. Temperature and pH were measured byusing standard method. The microbial analysis was done by serial dilution method and bacterial growth Nutrient agar(NA) and Czapek Dox Agar (CDA) for fungi enumeration. The pure cultures of the bacterial isolates were subjected tovarious morphological and biochemical characterization tests like, Catalase Test (CAT), Oxidase Test (OXT), IndoleTest (INT), Methyl Red Test (MRT), Voges-Proskauer Test (VPT), Citrate Utilization Test (CUT), Urease Test (URT),Nitrate Reduction Test (NRT), Hydrogen Sulphide Production (H2S), Starch Hydrolysis Test (SHT) and GelatineHydrolysis Test (GHT) to determine the identity of the bacteria isolates. The results reveal that, the temperature of thewindrows in all seasons reached maximum after 4 weeks of composting and then decreased by the end of thecomposting period (60 days). Marked changes in pH values of the composts in all seasons during degradation stageswere found, but final stages shows the pH was at neutral except rainy season (60 days old compost sample with 8.7).The microbial populations were significant increase during initial stages of composting process and final stagespathogenic microbes was reduced, for all the three seasons. The Bacillus Sp., Pseudomonas and Asprigillus Sp., wasdominate species during composting process. From the present investigation, it can be concluded that, the summer andautumn seasons microbial activities faster because the favourable environmental conditions for supporting the properwastes degradation, therefore, these two seasons for obtained better quality of compost than rainy season.