期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：9
页码：16199
DOI：10.15680/IJIRSET.2014.0309057
出版社：S&S Publications
摘要：Candida is a medically important fungi because of its high frequency as a commensal and pathogenicmicroorganism causing superficial as well as invasive infections. Because the accurate diagnosis of candidiasis remainsdifficult, a fast and reliable assay for characterization of fungal pathogens is critical for the early initiation of adequateantifungal therapy and/or for introduction of preventive measures. As novel molecular genetic techniques arecontinuously introduced, their role in the management of infectious diseases has also been growing .Strain typing anddelineation of the species are essential for understanding its biology, epidemiology and population structure. A widerange of molecular techniques have been used for this purpose including non-DNA-based methods (multi-locus enzymeelectrophoresis), conventional DNA-based methods (electrophoretic karyotyping, random amplified polymorphic DNA,amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep-PCR) andDNA-based methods called exact typing (PCR based methods, microstallite length polymorphism, multilocus sequencetyping, DNA microarray ) because they generate unambiguous and highly reproducible typing data. Today, molecularstrategies complement conventional methods and provide more accurate and detailed insight. It can be expected thatfuture technical development will improve their potential furthermore.In this article, we provide a critical review on thevigorously fermenting field of molecular approaches to Candida identification and typing and summarized theiradvantages and limitations with regard to their discriminatory power, reproducibility, cost and ease of performance.
