期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：10
页码：16886
DOI：10.15680/IJIRSET.2014.0310068
出版社：S&S Publications
摘要：An efficient protocol had been developed for the root culture of Luffa acutangula (L.) Roxb. The rootand leaf explants from 21 day old in vitro raised seedlings were cultured on half strength Murashige and Skoog (MS)medium supplemented with different concentrations and combinations of Indole-3-acetic acid(IAA), Indole-3-butyricacid (IBA) and Napthalene acetic acid (NAA). IBA at 1.0 mgl-1 combined with NAA (1.0 mgl-1) induced maximumpercentage of rooting from leaf explants, under total dark condition. After 3 weeks, well established roots wereseparated. Fresh root tissue from the leaf explants and root tips from in vitro seedlings were subcultured in half strengthMS liquid medium supplemented with different concentrations and combinations of IAA, IBA and NAA, undercontinuous agitation at 80 rpm under total dark condition. The biomass of root culture (derived from leaf explants) inthe MS medium fortified with IBA(1.0 mgl-1), NAA(1.0mgl-1) and L-glutamine (20 mgl-1) was increased to 5.38 g FWand the biomass of root culture (derived from root tip) in the MS medium supplemented with IBA (0.5 mgl-1), NAA(1.0 mgl-1) and L- glutamine (20 mgl-1) was increased to 5.82 g FW after 7 weeks of culture.
