期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：12
页码：17890
DOI：10.15680/IJIRSET.2014.0312021
出版社：S&S Publications
摘要：Fresh water dwelling fishes have scanty information on gut microflora associated with them and there is a paucity of kno wledge regarding microbial enzyme activity in fish gastrointestinal tracts. Although some of the enzymes like cellulase, proteases have been reported to be associated in the gut of fish.Amylase is one of the most important enzymes known and is of great significance and contributes of about 25% of the total enzyme market. It has a number of potential applications in food, pharmaceutical and fine chemical industries. This work describes the isolation, characterisation and optimization of amylase producing micro-organisms from gastrointestinal tract ofCatla catla. Biological production of amylase by bacterial isolate PAC4 isolated from fish gut was identified to be Bacilluscereus. The maximum amylase production was reached to 92+ 3 U/ml in 48 hours of incubation.The various physico-chemical factors for optimum amylase fermentationwere found to be 3% sodium chloride concentration,37 °C temperature and pH 7.
关键词：Catla catla; Bacillus cereus; amylase; fermentation; media optimization
