摘要:The aims of this research were to know the concentration of arrowroot flourwhich produced the highest reducing sugar in saccharification process byA. niger, as well as to know the efficiency of ethanol production by Z.mobilis. The framework of this research was the increasing needs ofethanol; meanwhile ethanol could be obtained from the fermentationarrowroot. This research was carried out in two stages. In thesaccharification stage, there are four concentrations, namely: 10%, 20%, 30%and 40%, and A. niger concentration of 10% (v/v) for each arrowroot flourconcentration with spores amount 3,3x106/mL. The parametric measurementincludes: starch concentration in the starting day and the sixth day, whilethe reducing sugar concentration and pH was measured every 24 hoursduring 6 days. In the ethanol fermentation, Z. mobilis concentration havebeen used 10% (v/v) with cells amount 5,1x107/mL and it was used insolution product of arrowroot starch saccharification and parametricmeasurement carried out during 72 hours includes: ethanol and reducingsugar concentration and the growth of Z. mobilis cells. The data resultedfrom parametric measurement. The result of the research showed thatarrowroot flour concentration of 10% produced the highest reducing sugarin process of saccharification with reducing sugar concentration was 1,230g/100 mL in four days and the efficiency of ethanol production by Z. mobilisduring 72 hours was 83,03%.