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  • 标题:Perbedaan Metode ELISA Sandwich A dan B dalam Deteksi Antigen Membran Toxoplasma gondii
  • 本地全文:下载
  • 作者:MURKATI ; NOERHAYATI SURIPTO ; SUPARGIYONO
  • 期刊名称:Bioteknologi
  • 印刷版ISSN:0216-6887
  • 出版年度:2004
  • 卷号:1
  • 期号:2
  • 页码:54-57
  • DOI:10.13057/biotek/c010205
  • 出版社:UNS Solo
  • 摘要:Spreading of toxoplasmosis to fetus can by placenta, so it caused theabortion, born dead or congenital defect. To diagnosis this disease for fixedthe acute infection must get the significant increasing of IgG by the soft fee.The objections of this study are to know the difference between ELISASandwich A and B in detecting of membrane antigen of Toxoplasma gondii(T. gondii) in placenta tissue of pregnant women three-semester I and IIwith spontaneous abortion in Surakarta. One hundred serum and placentatissue samples of pregnant women three-semester I and II withspontaneous abortion are got from dr. Muwardi Hospital. IgM anti Toxofrom serum was examined by Toxo ISAGA Kit and IgG anti Toxo by ToxoScreen DA Kit. Detecting of membrane antigen of T. goodie from placentatissue were done by ELISA Sandwich A and B. The result of thisexperiment showed that 33% were positive IBM and or Gig anti Toxo.Detection of membrane antigen toward 33 samples with positive Toxo (IgGpositive) was highly significant different between ELISA Sandwich A (3%positive) toward ELISA Sandwich B (72.7% positive).
  • 关键词:Membrane antigen; Toxoplasma gondii; placenta aborted;women; ELISA.
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