摘要：Rice bran was produced from rice-hulling process. Rice bran was containhigh starch, therefore it was used to saccharification process. The aim ofthis research was to study the optimum concentration of rice bran toproduce reducing sugar on saccharification process by Rhizopus oryzae. The100 mL-rice bran medium (5, 10, 15, 20, 25, and 30%) were sterilized at 1210C,for 15 minutes and inoculated with R. oryzae (± 2x106 cfu) and incubated for3 days. The concentration of reducing sugar, starch, pH value, and biomasswere analyzed everyday. Concentration of starch was decreasing duringsaccharification process. However, the concentration of reducing sugar wasmaximum at first day saccharification. The optimum concentration of ricebran for saccharification process was showed by maximum production ofreducing sugar (15,347 mg/mL); it was in 20% concentration of medium. ThepH value was decreasing during saccharification process, and the biomasswas reversed
