摘要：Mud crab is the most commercial crabs which are highly sold because oftheir delicious taste and high protein content. In nature, the captureintensity of this crab is very high so the population would decrease rapidly.For this reason people start to breed it. At the moment breeding of mudcrab still have a lot of problems. One of those is the lower survival rate oflarva stage due to the diseases. The objective of this research was to observethe sensitivity of mud crab from various stages to White Spot SyndromeVirus (WSSV). The research was conducted in Pathology laboratory, BalaiBesar Riset Perikanan Budidaya Laut Gondol, Bali, January to April 2004.This experiment was run using three stages they were zoea-1, zoea-3 andcrablet stage. The treatment of this research were: for zoea-1 and zoea-3stage, i.e. treatment (A) the larvae were bathed for 2 hours in stock WSSVsuspension, in which the solution was diluted for 150 times; treatment (B)the larvae were bathed for 2 hours in stock WSSV suspension, in which thesolution was diluted for 15000 times; C as control, without WSSV. Forcrablet stage the WSSV infection was done by feeding them with tigershrimp which infected by WSSV. ANOVA used to analyze the differenteffect of infection treatment, i.e. diluted 150x, 15000x, and control tosurvival rate, then continue DMRT test in 5% level. T test used to comparetwo treatments i.e. infection treatment with feed and without infection.PCR method used to detect WSSV of infection in zoea-1, zoea-3 and crabletstages. The results showed that for zoea-1 and zoea-3 stage, increasingconcentration virus showed significant difference respect, showed bydecreasing of survival rate. In crablet stage the WSSV infection by feedingcan decrease survival rate. In proving the dead of mud crab zoea-1, zoea-3and crablet were due to the infection of WSSV, the PCR method was used.The result showed that look PCR product was revenged in the cell with sizeof band were as expractise. According to this result can be concluded thatzoea-1, zoea-3, and crablet stages of tested mud crab were sensitive toWSSV infection
