摘要：The aim of this research was to know effect of methyl jasmonate additionin any concentration on the callus growth and alkaloid reserpineproduction from in vitro culture of pule pandak [Rauvolfia serpentina (L.)Bentham ex. Kurz.]. The research frame based on the advantage of pulepandak as medicine plants sources. The addition of methyl jasmonate onthe callus medium would inhibit the callus growth. It also has effects forthe synthesis stricthosidine synthase. This enzyme had essential role on thereserpine biosynthesis. The research was conducted of two steps. First,initiation step to induct the callus formation. Second, treatment step toinduct reserpine production. The research was done by designing a factorrandom completed, that is methyl jasmonate concentration. Methyljasmonate concentration which is 0 μM, 10 μM, 20 μM, 40 μM, 80 μM, and100 μM. The result data was qualitative form, that was callus morphologyand quantitative form, fresh weight and dried weight, and reserpinecontent. The analysis of data used the ANOVA and DMRT 5%. The resultof this research indicated that increasing of methyl jasmonate additionreduced the callus growth. The more methyl jasmonate concentration (10,20, 40, 80 μM) was added, the higher reserpine was produced. Methyljasmonate concentration in 80 μM produced the highest reserpine contentthat is 0.75 mg/g
