摘要：The development of new medicines as immunostimulator agent has beingincreased according to the development of lethal diseases, for exampletumor that suppress the immune responses. Propolis, which is resinoussubstance produced by honeybees known to have antimicrobial, antiinflammatory,antioxidant, immunostimulator, and anti-tumor activities.The aims of this experiment were to study the effect of propolis asimmunostimulator agent in proliferation of mice-suffered with mammarytumor T lymphocyte and the mammary-tumor cells’ viability after beentreated with stimulated T lymphocyte. Three different dozes of propolisused in this experiment were 0.5 μg/mL, 1.5 μg/mL, 4.5 μg/mL, and added incell culture to measure the proliferation of T lymphocyte. Proliferationactivity of T lymphocyte was examined with 3-(4.5-dimethyl-2-thiazolyl)-2.5-diphenyl-2H-tetrazolium bromide (MTT) assay. Data was analyzed byusing Analysis of Variance (ANOVA) then followed by Duncan MultipleRanges Test (DMRT) 5%. The result showed that the proliferation of Tlymphocyte was increased after addition of propolis. The highest OD of Tlymphocyte was reached in addition of propolis 4.5 μg/mL (2.363). In theexamination of mice-mammary tumor cells’ viability, stimulated Tlymphocyte was added to tumor cells culture and the results showed thatthe viability of tumor cells was decreased according to the increasing of Tlymphocyte proliferation. The lowest tumor cells’ viability was reached inaddition of stimulated T lymphocyte with propolis 4.5 μg/mL (4.667).
关键词：immunostimulator; OD of T lymphocyte; propolis; tumor cells;viability; T lymphocyte
