摘要:The objectives of this research were to find out the increasenumber of two bacterials populations, Azotobacter and Azospirillum, due to the useof manure fertilizer. The exsperiment was conducted using group randomly designedwith two treatments. The plant populations were treated (i) whithout fertilizer as thecontrol, (ii) with manure fertilizer, and (iii) with NPK fertilizer. Data wasexperimentally collected by planting chili in several plots treated by manure, withthree replications. The field experiment was conducted in Gathak Village,Karangnongko Sub-district, Klaten District, Central Java. The data collected consistof the total population of Azotobacter and Azospirillum, nitrogen content in soil andthe chili yield. The primary data of research were analyzed using ANOVA test andfollowed by LSD test, with the degree of significance by 95% .The results showedthat the manure fertilizer can increase the population of bacteria as many as 0.02%(Azotobacter) and 0.46% (Azospirillum) when they were compared to the control one.So that it can increase the soil fertility when they were used in long time. Thereforeincreasing the nutrient availability in the soil was occurred. Application of manurefertilizer could increase the total nitrogen content in the soil and it is very useful forthe fertilizing of plants.