摘要:The aims of this research are to know the potency of Rhizopus oligosporus andRhizopus oryzae as a coagulant in tofu processing for increasing the amount oflinoleic and linolenic acids, and to know the time that needed by R. oligosporus andR. oryzae for increasing the amount of linoleic and linolenic acids. It uses PDA forinoculating fungi, and it is done at Sub-Lab Chemistry, Central Laboratory forMathematics and Natural Sciences, Sebelas Maret University, Surakarta. The tofumaking was done in “Dele Emas” Tofu Factory, Surakarta. Analysis of linoleic andlinolenic acids were done by Gas Chromatography, in LPPT-UGM Yogyakarta. Theconclusion of this research are R. oligosporus dan R. oryzae having a potency as acoagulant in tofu processing for increasing the amount of linoleic and linolenic acids.R. oryzae needs 18 hours to coagulate the tofu, and R. oligosporus needs 12 hours forthe same process. The highest amount of linoleic and linolenic acids were obtainedby R. oryzae at 6 hours of fermentation (0.26% and 0.14%), and 24 hours offermentation by R. oligosporus (0.06% and 0.04%).