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  • 标题:Clonal propagation of Yucca aloifolia L.
  • 本地全文:下载
  • 作者:Pavel Karpov
  • 期刊名称:Environmental and Experimental Biology
  • 印刷版ISSN:1691-8088
  • 出版年度:2004
  • 页码:177-182
  • 出版社:University of Latvia
  • 摘要:The optimal nutrient media and concentrations of growth regulators for obtaining Yucca aloifolia L. plants from seed culture, axillary branching and rooting of microshoots were determined. The common duration of germination of seeds of yucca in vitroon the hormone-free Monnier medium was 152 days to achieve 100 % germination. For the fi rst time, modifi ed Quorin and Lepover medium supplemented with 6-benzylaminopurine (1.5 mg l-1) and naphthalene acetic acid (0.06 - 0.1 mg l-1) was used for adventive shoot formation (7 to 8 shoots per explant) in Y. aloifoliaepicotyl culture. The effects of indole-3-acetic acid, naphthalene acetic acid indole-3-butyric acid, 2.4-dichlorophenoxyacetic acid and its combinations on root proliferation were determined. The best rooting of microshoots of Y. aloifoliawas found on Murashige and Skoog medium supplemented with 1 mg l-1indole-3-butyric acid and light intensity 800 to 1200 lux. The rate of regenerant survival reached 87.1 % in a soil mixture of garden soil / peat / sand (2:1:1). The obtained results were useded to develop a the scheme of clonal propagation of Y. aloifolia
  • 关键词:growth regulators; in vitropropagation; yucca
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