摘要：Plant hypersensitive response (HR) is a highly controlled reaction to prevent spread of biotroph pathogens. Understanding the mechanisms of HR could potentially allow development of crop plant varieties with improved resistance to pathogens. HR and its molecular control mechanisms have been extensively studied in model organisms, such as Arabidopsis thaliana, allowing to identify numerous necrotic or lesion-mimic mutants that constitutively express the HR even in absence of pathogen. Arabidopsis LSD1gene encodes a zinc fi nger protein characterized as a central regulator of HR. Here we identifi ed three genes homologous to the LSD1in barley and compared them to LSD1homologues in other grass species. Barley gene CBC04043appeared to be more similar to the Arabidopsis LOL1gene, however, the two other genes originating from gene duplication aft er the monocot - dicot divergence represented candidate genes for the LSD1orthologue. As the sequence analysis alone did not allow identifi cation of the LSD1orthologue in barley, expression of the three genes was studied in barley necrotic mutants nec1and nec3
