期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2016
卷号：5
期号：4
页码：5797
DOI：10.15680/IJIRSET.2016.0504096
出版社：S&S Publications
摘要：Laccase enzymes extensively produced by white-rot fungi are evaluated in the field of biotechnologyresearch due to its unique properties such as high reaction rates, broad substrate specificity and oxygen as aneconomical co-factor. The intracellular and extracellular laccase was obtained from white-rot fungus Schizophyllum sp.by liquid state fermentation. The intracellular laccase possess higher laccase activity in the 8th day of reaction andshowed a decline from the 10th day. The isoenzymes were purified by ion-exchange and size exclusion chromatography.The native gel analysis rendered only one single band in the purified intracellular culture filtrate.
