期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2016
卷号：5
期号：6
页码：10931
DOI：10.15680/IJIRSET.2015.0506249
出版社：S&S Publications
摘要：Cation-exchange chromatography was performed in conjunction with fast protein liquid chromatography(FPLC) on 70% ethanol soluble crude wheat gliadin by using strongly HiPrep SP Sepharose HP 16/10 column. Twodifferent buffers were used for column equilibration and protein binding, buffer A (2 M urea; 0.05 M sodium acetatepH 4.6)and buffer B (2 M urea; 0.05 M sodium acetate; 0.2 M NaCl pH 4.6), respectively.Wheat gliadin sample wasdissolved in buffer Aand column was equilibrated in respective buffers, after binding eluted via NaClconcentrationgradient of buffer IB(2 M urea; 0.05 M sodium acetate; 0.2 M NaCl pH 4.6). Cation-exchange chromatogram wassegregated into total 14 peaks.
