期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：5
页码：1337
出版社：S&S Publications
摘要：Nucleic acid, proteins and their degradative enzymes (DNase omitted) associated with adventitious rootformation (ARF) were determined in different phases at the base of hypocotyl cuttings of mung bean (Vigna radiata L.cv. 105). DNA and RNA contents in mung bean hypocotyls showed that there was a marked change in nucleic acidcontents (DNA and RNA) during the early inductive phase (0-24h). However, there was a gradual accumulation ofDNA and RNA after 24h up to the early expression phase (24-96h). Although the rise in the content of RNA wasmarkedly greater than that of DNA. The synthesis of new DNA and RNA might have a role during new root initialformation in mung bean hypocotyls. The pattern of RNase activity was inversely correlated with endogenous RNAcontent during the induction and early initiation phase (0-48h). The total protein contents tally with RNA contentsuggesting that high protein synthesis is preceded by RNA synthesis. Soluble protein increased during early phase (0-72h) but insoluble protein was remarked low during this early phase. However, the greater activities of proteases(acidic and neutral) at the expression phase (72-120h) rather than at the induction phase (0-24h) may indicate that theyare involved in protein turnover during root development at the later phase in mung bean hypocotyls explants.
关键词：adventitious root; mung bean; nucleic acids (DNA and RNA); protease; protein.
